Two-color fluorescence in situ hybridization (FISH) in combination with digital image analysis was used to develop an automatic system for the detection and classification of chromosome aberrations. Algorithms were developed for the automatic thresholding of the three digitized images: an FITC image
Use of chromosome painting for detecting stable chromosome aberrations induced by melphalan in mice
โ Scribed by Antonella Sgura; Laura Stronati; Francesca Gullotta; Andrea Pecis; Serena Cinelli; Antonella Lascialfari; Caterina Tanzarella; Francesca Pacchierotti
- Publisher
- John Wiley and Sons
- Year
- 2005
- Tongue
- English
- Weight
- 112 KB
- Volume
- 45
- Category
- Article
- ISSN
- 0893-6692
- DOI
- 10.1002/em.20107
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The development of 24-colour fluorescence in situ hybridisation (FISH) has led to significant advances in cytogenetic research and offers the potential for automated karyotypic analysis. However, these techniques are not in routine research or clinical use because of limitations in methods of probe
Cytochalasin B-blocked binucleated human lymphocytes from a healthy male donor were used to detect micronucleus induction and other aneuploidy events (chromosome loss and gain) after treatment with griseofulvin (GF), estramustine (EM), and sodium orthovanadate (Na 3 VO 4 ). A two-color FISH was perf