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Detection of chromosome loss and gain induced by griseofulvin, estramustine, and vanadate in binucleated lymphocytes using FISH analysis

✍ Scribed by Lucia Migliore; Laura Zotti-Martelli; Roberto Scarpato

Publisher: John Wiley and Sons
Year: 1999
Tongue: English
Weight: 78 KB
Volume: 34
Category: Article
ISSN: 0893-6692
DOI: 10.1002/(sici)1098-2280(1999)34:1<64::aid-em11>3.0.co;2-3

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✦ Synopsis

Cytochalasin B-blocked binucleated human lymphocytes from a healthy male donor were used to detect micronucleus induction and other aneuploidy events (chromosome loss and gain) after treatment with griseofulvin (GF), estramustine (EM), and sodium orthovanadate (Na 3 VO 4 ). A two-color FISH was performed by using centromeric probes for chromosome 2 (FITC labeled) and the X chromosome (TRITC labeled) to measure chromosome loss and gain events in binucleated cells. GF induced mainly aneuploid binucleates involving the X chromosome, but this was not associated with preferential loss of one of the two chromosomes. EM preferentially induced aneuploidy of chromosome 2, and Na 3 VO 4 of the X chromosome. Our results indicate that chromosome malsegregation events (chromosome loss and/or gain) are probably not randomly induced, suggesting that different mechanisms leading to aneuploidy may be either chromosome-dependent or compound-and doserelated.

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