𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Novel method for the production of multiple colour chromosome paints for use in karyotyping by fluorescence in situ hybridisation

✍ Scribed by Ian Roberts; Johannes Wienberg; Elisabeth Nacheva; Colin Grace; Darren Griffin; Nicholas Coleman

Publisher
John Wiley and Sons
Year
1999
Tongue
English
Weight
467 KB
Volume
25
Category
Article
ISSN
1045-2257

No coin nor oath required. For personal study only.

✦ Synopsis

The development of 24-colour fluorescence in situ hybridisation (FISH) has led to significant advances in cytogenetic research and offers the potential for automated karyotypic analysis. However, these techniques are not in routine research or clinical use because of limitations in methods of probe preparation. This article presents new probe construction protocols and strategies for multiple-colour karyotyping by chromosome painting, which makes the technique more efficient and may lead to more widespread implementation. We used paints generated by our protocols to demonstrate the presence of a cryptic translocation t (13;11;22) in the paediatric sarcoma cell line RMS 1598.

πŸ“œ SIMILAR VOLUMES

Novel and Highly Effective Method for th
Novel and Highly Effective Method for the Trimethylsilylation of Alcohols and Phenols with Hexamethyldisilazane (HMDS), Catalyzed by I2 Generated in situ Using Fe(NO3)3β‹…9 H2O/NaI under Heterogeneous and Neutral Conditions
✍ Ardeshir Khazaei; Sadegh Rahmati; Amin Rostami πŸ“‚ Article πŸ“… 2009 πŸ› John Wiley and Sons 🌐 German βš– 152 KB πŸ‘ 1 views

## Abstract Structurally diverse alcohols and phenols were trimethylsilylated in a clean and efficient reaction with hexamethyldisilazane (HMDS) based on the use of I~2~ generated __in situ__ from Fe(NO~3~)~3~β‹…9β€…H~2~O/NaI. The reaction occurs very rapid in good‐to‐high yield in CH~2~Cl~2~ at room t