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Use of a volatile buffer system in ion-exchange high-performance liquid chromatography of oligonucleotides

✍ Scribed by Janet M. Munholland; Karen A. Bright; Ross N. Nazar

Publisher
Elsevier Science
Year
1989
Tongue
English
Weight
428 KB
Volume
178
Category
Article
ISSN
0003-2697

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Cation-exchange high-performance liquid
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✍ A.J.M. van den Eijnden-van Raaij; I. Koornneef; Th.M.J. van Oostwaard; S.W. de L πŸ“‚ Article πŸ“… 1987 πŸ› Elsevier Science 🌐 English βš– 546 KB

The chromatographic behavior of a number of globular proteins was studied on a Bio-Sil TSK CM-2-SW weak cation exchange HPLC column under acidic conditions. A linear gradient of O-I M NH4Ac in I M HOAc, inducing a convex pH gradient from 2.4-4.8, resulted in an excellent separation of highly basic p