Purification of micromolar quantities of nucleotide analogs by reverse-phase high-performance liquid chromatography using a volatile buffer at neutral pH

Separation of nucleosides and nucleotide

Separation of nucleosides and nucleotides by reversed-phase high-performance liquid chromatography with volatile buffers allowing sample recovery

✍ Charlotte Yu Ip; Daniel Ha; Paul W. Morris; Mark L. Puttemans; Duane L. Venton 📂 Article 📅 1985 🏛 Elsevier Science 🌐 English ⚖ 479 KB

Reversed-phase high-performance liquid chromatography using a C,8 column with volatile buffers as the eluant was applied to the separation of a number of nucleosides and nucleotides. Groups of seven nucleosides and five nucleoside monophosphates were separated isocratically employing 0.1 M trimethyl