Volatile buffer system for high-performance anion-exchange chromatography of nucleotides
โ Scribed by Ute Linz
- Publisher
- Elsevier Science
- Year
- 1983
- Tongue
- English
- Weight
- 183 KB
- Volume
- 260
- Category
- Article
- ISSN
- 1873-3778
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Several types of high-performance silica-based supports have been found to be effective in the separation of polynucleotides. The principal difference in these materials is the type of bonded phase and the method by which it is attached to the silica support. One approach is the coupling of stationa
Oligosaccharides released enzymatically by N-glycanase from fetuin, alpha-acid glycoprotein, human chorionic gonadotropin, platelet-derived growth factor, and kallikrein were chromatographed on a polymeric pellicular anion-exchange column at pH values of 5 and 13. Separations occurred into groups of
Reversed-phase high-performance liquid chromatography using a C,8 column with volatile buffers as the eluant was applied to the separation of a number of nucleosides and nucleotides. Groups of seven nucleosides and five nucleoside monophosphates were separated isocratically employing 0.1 M trimethyl