Uricase based methods for determination of uric acid in serum
โ Scribed by Zhao, Yunsheng ;Yang, Xiaoyan ;Lu, Wei ;Liao, Hong ;Liao, Fei
- Publisher
- Springer-Verlag
- Year
- 2008
- Weight
- 141 KB
- Volume
- 164
- Category
- Article
- ISSN
- 0344-838X
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We developed and evaluated an assay for serum uric acid based on the uricase (EC 1.7.3.3)-catalase (EC 1.11.1.6)formaldehyde dehydrogenase (FADH, EC 1.2.1.46) method coupled with formate dehydrogenase (formate:NAD oxidoreductase, FDH, EC 1.2.1.2). Formate dehydrogenase from Pseudomonas oxalaticus ca
A large number of methods for the determination of uric acid content in serum or urine have been reported in the literature. However, precise methods for the determination of the content of uric acid particularly in viscera have not been developed yet and considerable variations among the values obt