A large number of methods for the determination of uric acid content in serum or urine have been reported in the literature. However, precise methods for the determination of the content of uric acid particularly in viscera have not been developed yet and considerable variations among the values obtained in each method have been reported.
It was therefore appropriate in the present study to compare existing methods for the determination of uric acid content in viscera such as calorimetric, uricase, and precipitation methods which seem to be suitable for this purpose.
As a result of such investigations, the authors are suggesting the use of the technique combining precipitation and uricase methods.
Methods
Uric acid obtained from Sigma Chemical Company was recrystallized according to the method of Pudelkiewicz
( 1). The uricase used was Sigma Type II obtained from Sigma Chemical Company.
Chicken liver, kidney, spleen, muscle. heart, and aorta which had been frozen at -70ยฐC were used for the assays. c'0tt~puri.w~ oj'tlw three methods (colorimetric, uricase, atzdprec~ipitfltiorz)JOr ttlcosuritzg uric mid c'orltetit iri ,isccra. Viscera 36OO<q. The supernatant solution was used for the analysis.
Colorimrtric rrlrthod.