A double mutant being thermoconditionally defective in mutation induction as well as in repair of pre-lethal UV-induced DNA damage (rev2ts) and deficient in excision repair (rad3-2) was studied in temperature-shift experiments. The influence of inhibitors of DNA replication (hydroxyurea, aphidicolin
Uptake of DNA by fragile mutants ofSaccharomyces cerevisiae
โ Scribed by Pencho V. Venkov; Vesselin P. Ivanov
- Publisher
- Springer-Verlag
- Year
- 1982
- Tongue
- English
- Weight
- 227 KB
- Volume
- 5
- Category
- Article
- ISSN
- 0172-8083
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
Incubation of cdc8 mutants of the yeast Saccharomyces cerevisiae in YPD under permissive conditions, when DNA replication is taking place, prior to transfer to restrictive conditions, strongly stimulates induction of cdc+ colonies of ethyl methane sulphonate (EMS)- and methyl methane sulphonate (MMS
Blasticidin S-resistant mutants of S. cerevisiae were isolated and characterized. Resistant mutations were found to fall into two complementation groups. A single recessive nuclear gene was responsible for each group, donated as blsl and bls2, respectively. A gene blsl was linked to an ilv3 gene loc
A general procedure for the curing of 2-/~m in Saccharomyces cerevisiae is described. The method is based on the displacement of endogenous 2-~m DNA by the recombinant plasmid pMP78-1, which carries the yeast leu2 gene and the 2-/am DNA replicon, but cannot be maintained stably in a yeast cell witho