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Analysis of mutagenic DNA repair in a thermoconditional mutant ofSaccharomyces cerevisiae

โœ Scribed by Wolfram Siede; Friederike Eckardt

Publisher
Springer-Verlag
Year
1986
Tongue
English
Weight
736 KB
Volume
10
Category
Article
ISSN
0172-8083

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โœฆ Synopsis

A double mutant being thermoconditionally defective in mutation induction as well as in repair of pre-lethal UV-induced DNA damage (rev2ts) and deficient in excision repair (rad3-2) was studied in temperature-shift experiments. The influence of inhibitors of DNA replication (hydroxyurea, aphidicolin) was determined. Additionally, an analysis of the dose-response pattern of mutation induction ("mutation kinetics") at several ochre alleles was carried out. It was concluded that the UV-inducible REV2 dependent mutagenic repair process is not induced in excision-deficient cells. In excision-deficient cells, REV2 dependent mutation fixation is slow and mostly post-replicative though not dependent on DNA replication. The REV2 mediated mutagenic process could be separated from the repair function.

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