Using the thermoconditional yeast mutant rev2ts that controls an apparently site-specific step of mutagenic DNA repair it was possible to measure the time course of REV2 dependent UV-induced reversion of the ochre allele his5-2 and recovery of survival for UV-treated stationary phase cells: due to t
Analysis of mutagenic DNA repair in a thermoconditional mutant of Saccharomyces cerevisiae
โ Scribed by Siede, Wolfram ;Eckardt, Friederike
- Publisher
- Springer
- Year
- 1986
- Tongue
- English
- Weight
- 699 KB
- Volume
- 202
- Category
- Article
- ISSN
- 0026-8925
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
The time course of REV2 dependent recovery from prelethal UV damage and UV-induced locus-specific reversion of the his5-2 allele was determined in temperature-shift experiments by use of a thermoconditional allele of the rev2 gene (rad5-8, rev2ts). In UV-irradiated, exponentially growing rev2ts cell
A double mutant being thermoconditionally defective in mutation induction as well as in repair of pre-lethal UV-induced DNA damage (rev2ts) and deficient in excision repair (rad3-2) was studied in temperature-shift experiments. The influence of inhibitors of DNA replication (hydroxyurea, aphidicolin
The repair of UV-irradiated DNA of plasmid pBB29 was studied in an incision-defective rad3-2 strain of Saccharomyces cerevisiae and in a uvrA6 strain of Escherichia coli by the measurement of cell transformation. Plasmid pBB29 used in these experiments contained as markers the DNA of nuclear yeast g
A DNA replication mutant of yeast, cdc8, was found to decrease UV-induced reversion of lys2-1, arg4-17, tyr1 and ura1. This effect was observed with all three alleles of cdc8 tested. Survival curves obtained following UV irradiation in cdc8 rad double mutants show that cdc8 is epistatic to rad6, as
A single recessive nuclear gene mutation has been isolated from strain 123.1C of Saccharomyces cerevisiae which appears to be conditionally deficient in nuclear DNA metabolism. Growth of the mutant strain at the elevated temperature of 36 degree C results in rapid loss of cell viability. However, no