Analysis of mutagenic DNA repair in a thermoconditional repair mutant of Saccharomyces cerevisiae

✍ Scribed by Siede, Wolfram ;Eckardt, Friederike ;Brendel, Martin

• Publisher: Springer
• Year: 1983
• Tongue: English
• Weight: 465 KB
• Volume: 190
• Category: Article
• ISSN: 0026-8925
• DOI: 10.1007/bf00331069

✦ Synopsis

The time course of REV2 dependent recovery from prelethal UV damage and UV-induced locus-specific reversion of the his5-2 allele was determined in temperature-shift experiments by use of a thermoconditional allele of the rev2 gene (rad5-8, rev2ts). In UV-irradiated, exponentially growing rev2ts cells the REV2 dependent repair activity persists for up to 8 h at permissive temperature (23 degrees C), while the REV2 dependent mutagenic process is mostly completed within 2 h. The REV2 dependent process in exponentially growing cells is highly impaired by inhibition of protein synthesis. However, a REV2 dependent repair activity independent of de novo synthesis is detectable, even in the presence of up to 200 micrograms/ml cycloheximide, a response not found in stationary phase cells. Thus, the REV2 dependent process seems to be partially constitutive in exponentially growing cells. Additionally, exponentially growing rev2ts cells were considerably more UV-sensitive at restrictive temperature (36 degrees C) than were stationary phase cells.