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Characterization of blasticidin S — resistant mutants ofSaccharomyces cerevisiae

✍ Scribed by Junpei Ishiguro; Masazumi Miyazaki

Publisher
Springer-Verlag
Year
1985
Tongue
English
Weight
222 KB
Volume
9
Category
Article
ISSN
0172-8083

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✦ Synopsis

Blasticidin S-resistant mutants of S. cerevisiae were isolated and characterized. Resistant mutations were found to fall into two complementation groups. A single recessive nuclear gene was responsible for each group, donated as blsl and bls2, respectively. A gene blsl was linked to an ilv3 gene located on the right arm of chromosome X. The resistant phenotypes from both genes were not associated with ribosomes known to be target sites of Blasticidin S, when analyzed by poly(U)directed polyphenylalanine synthesis. The resistant mechanisms of the mutations are discussed in this paper.

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