𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Uptake and distribution of exogenous serum proteins in the cultured rat embryo

✍ Scribed by Rhinehardt, Ann E. ;Carey, Sean W. ;Young, Marian F. ;Klein, Norman W.

Publisher
John Wiley and Sons
Year
1984
Tongue
English
Weight
478 KB
Volume
232
Category
Article
ISSN
0022-104X

No coin nor oath required. For personal study only.

✦ Synopsis

Head fold stage rat embryos were cultured on a medium consisting of adult rat serum for 48 hr. During the final 10 or 20 hr of culture [35S]-methionine was added to the medium. Sera from visceral yolk sac blood vessels, exocoelomic cavity fluid, and amniotic sac fluid were analyzed by SDSpolyacrylamide gel electrophoresis followed by Amido Black staining and fluorography. Amido Black staining showed bands in all three embryonic fluid compartments which migrated at rates comparable to transferrin, alpha-fetoprotein, albumin, IgG, and hemoglobin. Fluorography, however, showed that radioactivity was restricted to alpha-fetoprotein, IgG, and hemoglobin. This suggested that a t least some transferrin and albumin were transferred intact from the exogenous medium to the embryonic fluid compartments.

📜 SIMILAR VOLUMES

Culture of 9½-day rat embryos in human s
Culture of 9½-day rat embryos in human serum supplemented and unsupplemented with rat serum
✍ Reti, Leslie L. ;Beck, Felix ;Bulman, Stephanie 📂 Article 📅 1982 🏛 John Wiley and Sons 🌐 English ⚖ 193 KB

## Abstract We attempted to grow explanted 9½‐day rat embryos in vitro in human serum but were unable to demonstrate normal growth as has been previously described. The embryos were consistently smaller, anaemic, and frequently deformed. These abnormalities were overcome by supplementing the human

Culture of postimplantation rat embryos
Culture of postimplantation rat embryos in rabbit serum for the identification of the growth factor in fractionated rat serum
✍ Usami, Makoto ;Nakaura, Shinsuke ;Kawashima, Kunio ;Tanaka, Satoru ;Takanaka, Ak 📂 Article 📅 1992 🏛 John Wiley and Sons 🌐 English ⚖ 498 KB 👁 1 views

## Abstract The growth factor for postimplantation rat embryos was investigated on the basis of the serum species‐specificity in supporting embryonic development in culture. We used rabbit serum as a basal medium for the culture of head‐fold stage rat embryos, and examined the effects of various fr

Distribution and gene expression of cyto
Distribution and gene expression of cytoskeletal proteins in two-cell rat embryos and developmental arrest
✍ Matsumoto, Hiromichi ;Jiang, Jin-yi ;Mitani, Disuke ;Sato, Eimei 📂 Article 📅 2002 🏛 John Wiley and Sons 🌐 English ⚖ 282 KB 👁 1 views

## Abstract Previous observations in rat two‐cell embryos suggested that distribution of microfilaments and microtubules are involved in developmental arrest. Therefore, we examined the distribution of cytoskeletal proteins, actin binding proteins, and microtubule‐associated proteins in rat two‐cel

Neuritic differentiation and synaptogene
Neuritic differentiation and synaptogenesis in serum-free neuronal cultures of the rat cerebral cortex
✍ De Lima, Ana D.; Merten, Marcus D.P.; Voigt, Thomas 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 968 KB

To better understand the dynamics of the cellular processes involved in early neocortical development, we studied the neuritic differentiation and synaptogenesis of dispersed neurons grown in serum-free cultures under a wide variety of culture conditions. Microtubuleassociated protein (MAP2), phosph

Performance of thiophilic adsorption chr
Performance of thiophilic adsorption chromatography in the purification of rat IgG2b monoclonal antibodies from serum- and protein-free culture supernatants
✍ María Eladia Argüelles; Marta Alonso; María del Mar García Suárez; Luis Barneo; 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 85 KB 👁 1 views

The usefulness of thiophilic adsorption chromatography for the purification of rat IgG2b monoclonal antibodies has been evaluated. This approach has not shown specificity for immunoglobulins; therefore, to minimize potential interferences, the purification was carried out from supernatants of hybrid