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Culture of 9½-day rat embryos in human serum supplemented and unsupplemented with rat serum

✍ Scribed by Reti, Leslie L. ;Beck, Felix ;Bulman, Stephanie

Publisher
John Wiley and Sons
Year
1982
Tongue
English
Weight
193 KB
Volume
223
Category
Article
ISSN
0022-104X

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✦ Synopsis

Abstract

We attempted to grow explanted 9½‐day rat embryos in vitro in human serum but were unable to demonstrate normal growth as has been previously described. The embryos were consistently smaller, anaemic, and frequently deformed. These abnormalities were overcome by supplementing the human serum with 10% rat serum. The latter possibly provides yet unidentified essential growth factors required by rat embryos that are not present in human serum.

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