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Up-regulation of MDC15 (metargidin) messenger RNA in human osteoarthritic cartilage

✍ Scribed by Beate B. Böhm; Thomas Aigner; Angelika Gehrsitz; Carl P. Blobel; Joachim R. Kalden; Harald Burkhardt

Publisher: John Wiley and Sons
Year: 1999
Tongue: English
Weight: 203 KB
Volume: 42
Category: Article
ISSN: 0004-3591
DOI: 10.1002/1529-0131(199909)42:9<1946::aid-anr21>3.0.co;2-e

No coin nor oath required. For personal study only.

✦ Synopsis

Objective:

The aim of the study was to investigate the messenger rna (mrna) expression of the disintegrin metalloproteinase mdc15 (metargidin, or adam-15) in normal and osteoarthritic (oa) articular cartilage.

Methods:

In situ hybridization experiments and reverse transcription-polymerase chain reaction (rt-pcr) were performed on tissue samples of adult normal and oa articular cartilage.

Results:

Mdc15 mrna could be detected in normal articular cartilage by rt-pcr using tissue-extracted total rna as a template. however, the mrna level remained below the sensitivity of in situ hybridization. in contrast, in situ hybridizations of oa cartilage revealed an intense staining with the mdc15-specific riboprobes. the extension of the analysis to chondrosarcomas showed a strong up-regulation of mdc15 mrna in these malignant transformed cells.

Conclusion:

Our results demonstrate a markedly strong up-regulation of mdc15 in adult oa and neoplastic cartilage compared with adult normal articular cartilage, indicating a potential role of the disintegrin metalloproteinase in cartilage remodeling.

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