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Unstable rII mutants of bacteriophage T4

โœ Scribed by C.E. Folsome


Book ID
118848701
Publisher
Elsevier Science
Year
1964
Tongue
English
Weight
243 KB
Volume
14
Category
Article
ISSN
0006-291X

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Revertants of double opal-mutants of bacteriophage T4 have been obtained. The properties of these revertants suggest that reversion of double opal-mutants is effected by the activity of some gent-suppressor appeared in the phage genome. Restriction of these revertants by streptomycin-resistant bacte

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The EcoRI digestion products of phage T4 DNA have been examined using a phage DNA transformation assay. A 2.6 X 10(6) Dalton fragment was found to contain the rII genes. This fragment was purified and then treated with HindIII endonuclease. The cleavage products were ligated to the vector plasmid pB