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Revertants of double Opal-mutants of bacteriophage T4

โœ Scribed by Pozdnlakov, V. N. ;Maisurian, A. N.


Publisher
Springer
Year
1976
Tongue
English
Weight
186 KB
Volume
149
Category
Article
ISSN
0026-8925

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โœฆ Synopsis


Revertants of double opal-mutants of bacteriophage T4 have been obtained. The properties of these revertants suggest that reversion of double opal-mutants is effected by the activity of some gent-suppressor appeared in the phage genome. Restriction of these revertants by streptomycin-resistant bacterial strains shows that the suppression of the opal-mutants is realized at translation.


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Lysis protein T of phage T4 is required to allow the phage's lysozyme to reach the murein layer of the cell envelope and cause lysis. Using fusions of the cloned gene t with that of the Escherichia coli alkaline phosphatase or a fragment of the gene for the outer membrane protein OmpA, it was possib