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Enhanced recovery of rII mutations in the absence of selection in a ligase-negative mutant of bacteriophage T4

โœ Scribed by Douglas A. Campbell; Lucy B. Rowe

Book ID
116036379
Publisher
Elsevier Science
Year
1972
Tongue
English
Weight
522 KB
Volume
15
Category
Article
ISSN
0027-5107

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A hotspot for transition mutations in th
A hotspot for transition mutations in the rIIB gene of bacteriophage T4
โœ Singer, Britta Swebilius ๐Ÿ“‚ Article ๐Ÿ“… 1984 ๐Ÿ› Springer ๐ŸŒ English โš– 729 KB

We have previously demonstrated that the sequence 5'TGGCAA 3' located at codons 32-33 of the rIIB gene of bacteriophage T4 is a hotspot for transition mutations (Nelson et al. 1981). Here I report the properties of the same TGGCAA sequence introduced into the gene at codons 11-12. The sequence is hi