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Unscheduled synthesis of DNA and poly(ADP-ribose) in human fibroblasts following DNA damage

✍ Scribed by McCurry, Lynette Salter ;Jacobson, Myron K.

Publisher
Wiley (John Wiley & Sons)
Year
1981
Tongue
English
Weight
226 KB
Volume
17
Category
Article
ISSN
0275-3723

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✦ Synopsis

Abstract

Unscheduled DNA synthesis has been measured in human fibroblasts under conditons of reduced rates of conversion of NAD to poly(ADP‐ribose). Cells heterozygous for the xeroderma pigmentosum genotype showed normal rates of UV induced unscheduled DNA synthesis under conditions in which the rate of poly(ADP‐ribose) synthesis was one‐half the rate of normal cells. The addition of theophylline, a potent inhibitor of poly(ADP‐ribose) polymerase, to the culture medium of normal cells blocked over 90% of the conversion of NAD to poly(ADP‐ribose) following treatment with UV or N‐methyl‐N′‐nitro‐N‐nitro‐soguanidine but did not affect the rate of unscheduled DNA synthesis.

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