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Unscheduled DNA synthesis testing of pesticides

โœ Scribed by Ann D. Mitchell; Douglas E. Robinson; Gery P. Sterling; Patricia A. McAfee; Michael D. Waters


Book ID
113249547
Publisher
Elsevier Science
Year
1978
Tongue
English
Weight
140 KB
Volume
53
Category
Article
ISSN
0165-1161

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We describe a cytophotometric assay for unscheduled DNA synthesis (UDS) in asynchronously growing cells. Monolayer cultures of human HEp-2 and mouse MCa-11 cells were incubated with the carcinogen methylmethane sulfonate (MMS), as well as with hydroxyurea and (3H)thymidine. Slides were prepared, and