Unscheduled DNA synthesis testing of environmental mutagens
β Scribed by A.D. Mitchell; M.D. Guinee; M.V. McMacken
- Book ID
- 119091584
- Publisher
- Elsevier Science
- Year
- 1975
- Tongue
- English
- Weight
- 63 KB
- Volume
- 31
- Category
- Article
- ISSN
- 0165-1161
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
We describe a cytophotometric assay for unscheduled DNA synthesis (UDS) in asynchronously growing cells. Monolayer cultures of human HEp-2 and mouse MCa-11 cells were incubated with the carcinogen methylmethane sulfonate (MMS), as well as with hydroxyurea and (3H)thymidine. Slides were prepared, and
Testicular cells of male mice were isolated, and the incorporation of tritium labeled thymidine into the DNA of the cells was estimated after exposure to various chemical mutagens. The normal semiconservative DNA synthesis was suppressed by the addition of hydroxyurea. Thirteen compounds were tested