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Unexpected improvement in stability and utility of cytochrome c by solution in biocompatible ionic liquids

✍ Scribed by Kyoko Fujita; Maria Forsyth; Douglas R. MacFarlane; Robert W. Reid; Gloria D. Elliott

Book ID
101720952
Publisher
John Wiley and Sons
Year
2006
Tongue
English
Weight
213 KB
Volume
94
Category
Article
ISSN
0006-3592

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✦ Synopsis

Abstract

Proteins generally are only stable in vitro for short periods of time. This results in challenges during isolation and purification of recombinant proteins and reduces the shelf life of protein‐based pharmaceuticals. Here we show that certain novel, biocompatible ionic liquids provide a stabilizing solvent for proteins, for example, cytochrome c, such that structure and activity are maintained even after 6 months of storage at room temperature. Normally, this protein would be rendered inactive after only 1 week in buffered aqueous solution. The effect of the ionic liquid solvent appears to be related to protection against hydrolysis. Β© 2006 Wiley Periodicals, Inc.

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