Underivatized steroids on persilanized capillary columns with non-vaporizing on-column injection

✍ Scribed by Kovarich, E. ;Munari, F.

• Publisher: John Wiley and Sons
• Year: 1982
• Tongue: English
• Weight: 381 KB
• Volume: 5
• Category: Article
• ISSN: 0935-6304
• DOI: 10.1002/jhrc.1240050402

✦ Synopsis

Abstract

Recent developments in the preparation of capillary columns by persilanization of the glass surfaces have resulted in highly inactive, heat‐stable columns. With this type of column certain steroid hormone classes can be analyzed without derivatization; these include the 17‐keto steroids, 11‐oxo compounds, estrogens, and progesterone metabolites. It has been found that good quantitative analysis calls for non‐vaporizing, on‐column injection, as normal vaporizing injection results in breakdown of thermolabile compounds such as estriol and 17‐OH progesterone metabolites. The gas chromatographic method described was used to obtain urinary steroid profiles.