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Uncertainty in the determination of the molecular weight of poly(A)-containing RNA

โœ Scribed by Michael C. MacLeod

Publisher
Elsevier Science
Year
1975
Tongue
English
Weight
581 KB
Volume
68
Category
Article
ISSN
0003-2697

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โœฆ Synopsis

Estimates of the weight average molecular weight of mouse myeloma cell cytoplasmic poly(A)(รท)RNA obtained by polyacrylamide-gel electrophoresis and by sucrose gradient sedimentation differ by a factor of two. This difference is not due to degradation of the RNA molecules during sedimentation nor to acid poly(A) double helix formation during electrophoresis. The difference is probably due, at least in part, to the presence of poly(A) sequences in the RNA molecules.

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