๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

Molecular weight determination of glyoxalated RNA by sedimentation centrifugation

โœ Scribed by Micheal A. Nash; Marjorie Johnson; John E. Knesek; James C. Chan; James L. East

Publisher
Elsevier Science
Year
1981
Tongue
English
Weight
733 KB
Volume
111
Category
Article
ISSN
0003-2697

No coin nor oath required. For personal study only.

โœฆ Synopsis

A rapid, reliable sedimentation centrifugation technique has been developed to measure the molecular weights of rather large glyoxalated RNAs. A distinctive feature of this method is that the glyoxalated RNAs can be analyzed in sucrose gradients containing no denaturant. This feature allowed us to compute the sedimentation coefficients of glyoxalated RNAs by a comparison with those of native, untreated RNA markers. These values then were used to obtain accurate molecular weight estimates by applying the linear log-log relation between the molecular weight of an RNA and its sedimentation -coefficient.

๐Ÿ“œ SIMILAR VOLUMES

Single-stranded RNA molecular weight and
Single-stranded RNA molecular weight and shape determination by differential pressure capillary viscometry, sedimentation velocity, and gel electrophoresis
โœ Deborah L. Freerksen; Paul C.-F. Shih; Julia F. Vasta-Russell; Robert A. Horlick ๐Ÿ“‚ Article ๐Ÿ“… 1990 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 644 KB

Determination of the size of a population of nucleic acids can be achieved by several distinct methods. Most of these methods are cumbersome and require complicated equipment or techniques. We demonstrate here the use of a differential pressure capillary viscometer for the rapid and simple determina

The molecular weight of a fast sedimenti
The molecular weight of a fast sedimenting MS2 RNA form
โœ H. Slegers; W. Fiers ๐Ÿ“‚ Article ๐Ÿ“… 1972 ๐Ÿ› Wiley (John Wiley & Sons) ๐ŸŒ English โš– 51 KB

The Molecular Weight of a Fast Sedimenting MS2 RNA Form H. SLEGERS and W. FIERS [Article in Biopolymers, 9, 1373 (1970)l I n a previous publication we described conditions for the formation of a fast sedimenting structure formed by the reaction of formaldehyde with MS2 RNA a t acidic pH.1 The molec

Effect of Centrifuge Speed on the Sedime
Effect of Centrifuge Speed on the Sedimentation of High-Molecular-Weight Bacteriophage G DNA
โœ Frank Krasin ๐Ÿ“‚ Article ๐Ÿ“… 1979 ๐Ÿ› Wiley (John Wiley & Sons) ๐ŸŒ English โš– 236 KB ๐Ÿ‘ 1 views

## Abstract Large molecular weight bacteriophage G DNA, about five times larger than T2 DNA, was used to test Zimm's theory [(1974) __Biophys. Chem.__ **1**, 279โ€“291] for the effect of rotor speed on the sedimentation of large linear monodisperse DNA. Sedimentation profiles from neutral sucrose gra