A 9S RNA fraction from mouse reticulocytes, containing the active Ξ±- and Ξ²-globin mRNAs, has been isolated by hybridization of the polyadenylate regions in the mRNAs to oligo(dT)-cellulose. The adenylate-rich sequence isolated by limited RNase digestion of the globin mRNAs migrates between 4S and 5S
The size of poly(A)-containing RNAs inDrosophila melanogasterembryos
β Scribed by Mary M. Lamb; Charles D. Laird
- Publisher
- Springer
- Year
- 1976
- Tongue
- English
- Weight
- 764 KB
- Volume
- 14
- Category
- Article
- ISSN
- 0006-2928
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β¦ Synopsis
The size range of poly(A)-containing RNA from Drosophila melanogaster embryos has been estimated by hybridization with 3H-labeled poly(U) and subsequent fractionation on sucrose gradients. The median size of nuclear poly(A)-containing RNA is about 30 S (6000 nueleotides), and the median size of cytoplasmic poly(A)-containing RNA is about 17 S (1800 nucleotides). The relationship of these sizes to messenger RNA needed to code for protein and to the length of DNA contained in a chromomere is discussed.
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## Abstract Cytoplasmic polyβA containing RNA, synthesized by cultured chick embryo cells, was examined during growth in 5βbromodeoxyuridine. The kinetics of ^3^Hβadenosine incorporation into this species of RNA, when compared to the rest of the cytoplasmic RNA, and to control cells, indicates that