✦ LIBER ✦

Unambiguous Correlations of Backbone Amide and Aliphatic Gamma Resonances in Deuterated Proteins

✍ Scribed by Scott A. McCallum; T.K. Hitchens; Gordon S. Rule

Publisher: Elsevier Science
Year: 1998
Tongue: English
Weight: 267 KB
Volume: 134
Category: Article
ISSN: 1090-7807
DOI: 10.1006/jmre.1998.1525

No coin nor oath required. For personal study only.

✦ Synopsis

Two 3D NMR pulse sequences that correlate aliphatic gamma carbon resonance frequencies to amide proton and nitrogen chemical shifts in perdeuterated proteins are presented. The HN(CO-CACB)CG provides only interresidue connectivities (NH (i) and C ␥(i-1) ) while the HN(CACB)CG detects both the inter-and intraresidue (NH (i) and C ␥(i) or C ␥(i؊1) ) correlations. These two experiments are useful for sequential assignments and the identification of residue type from the C ␥ shifts. Spectra acquired on a perdeuterated 53-kDa protein illustrate the sensitivity and utility of these experiments.

📜 SIMILAR VOLUMES

Correlation of Backbone Amide and Side-C

Correlation of Backbone Amide and Side-Chain 13C Resonances in Perdeuterated Proteins

✍ Frank Löhr; Heinz Rüterjans 📂 Article 📅 2002 🏛 Elsevier Science 🌐 English ⚖ 177 KB

Side-chain carbon resonance assignments are difficult to obtain for larger proteins. While standard methods require protons for excitation and detection of magnetization, their presence is often unacceptable and often leads to unacceptable relaxation losses at the directly bound carbon sites. In thi

An HCCNH Triple-Resonance Experiment Usi

An HCCNH Triple-Resonance Experiment Using Carbon-13 Isotropic Mixing for Correlating Backbone Amide and Side-Chain Aliphatic Resonances in Isotopically Enriched Proteins

✍ B.A. Lyons; G.T. Montelione 📂 Article 📅 1993 🏛 Elsevier Science 🌐 English ⚖ 278 KB

Correlation of Backbone Amide and Alipha

Correlation of Backbone Amide and Aliphatic Side-Chain Resonances in 13C/15N-Enriched Proteins by Isotropic Mixing of 13C Magnetization

✍ S. Grzesiek; J. Anglister; A. Bax 📂 Article 📅 1993 🏛 Elsevier Science 🌐 English ⚖ 389 KB

We thank Claude Klee for help and encouragement in our study of calcineurin B, Hao Ren for protein expression, and Marius Clore and Dennis Torchia for useful discussions. This work was supported by the AIDS Targeted Anti-Viral Program of the Office of the Director of the National Institutes of Healt

Improved NMR Experiments for the Correla

Improved NMR Experiments for the Correlation of Backbone-Carbon with Aliphatic-Proton Resonances in Proteins

✍ F. Lohr; H. Ruterjans 📂 Article 📅 1995 🏛 Elsevier Science 🌐 English ⚖ 458 KB

An Efficient HN(CA)NH Pulse Scheme for T

An Efficient HN(CA)NH Pulse Scheme for Triple-Resonance 4D Correlation of Sequential Amide Protons and Nitrogens-15 in Deuterated Proteins

✍ Takahisa Ikegami; Shigeo Sato; Markus Wälchli; Yoshimasa Kyogoku; Masahiro Shira 📂 Article 📅 1997 🏛 Elsevier Science 🌐 English ⚖ 140 KB

The development of multidimensional triple-resonance i.e., the delays and field strengths of the RF pulses. The coherence flow can be described by NMR techniques has enabled us to study the solution structures of proteins with molecular masses smaller than 25 kDa (1-3). Among the experiments, the th

Correlation of the15N(i+ 1),13Cα(i), and

Correlation of the15N(i+ 1),13Cα(i), and1Hα(i) Backbone Resonances in13C/15N-Labeled Proteins by the (CO)N(CO)CAH Experiment

✍ Klaas Dijkstra; Gerard J.A. Kroon; Eiso AB; Ruud M. Scheek; Johan Kemmink 📂 Article 📅 1997 🏛 Elsevier Science 🌐 English ⚖ 107 KB

Recently, a novel three-dimensional, triple-resonance ex-respect to the nitrogen, which then evolves into in-phase periment was described which correlates intraresidue 13 CO, carbonyl magnetization again. Evolution under the 1 J CaCO 13