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An Efficient HN(CA)NH Pulse Scheme for Triple-Resonance 4D Correlation of Sequential Amide Protons and Nitrogens-15 in Deuterated Proteins

✍ Scribed by Takahisa Ikegami; Shigeo Sato; Markus Wälchli; Yoshimasa Kyogoku; Masahiro Shirakawa


Publisher
Elsevier Science
Year
1997
Tongue
English
Weight
140 KB
Volume
124
Category
Article
ISSN
1090-7807

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✦ Synopsis


The development of multidimensional triple-resonance i.e., the delays and field strengths of the RF pulses. The coherence flow can be described by NMR techniques has enabled us to study the solution structures of proteins with molecular masses smaller than 25 kDa (1-3). Among the experiments, the three-dimensional HN(CA)NNH and H(NCA)NNH methods are extremely beneficial for determining sequential connectivities between

N H (i Ϫ 1) H N (i Ϫ 1).