𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Correlation of Backbone Amide and Aliphatic Side-Chain Resonances in 13C/15N-Enriched Proteins by Isotropic Mixing of 13C Magnetization

✍ Scribed by S. Grzesiek; J. Anglister; A. Bax


Publisher
Elsevier Science
Year
1993
Tongue
English
Weight
389 KB
Volume
101
Category
Article
ISSN
1064-1866

No coin nor oath required. For personal study only.

✦ Synopsis


We thank Claude Klee for help and encouragement in our study of calcineurin B, Hao Ren for protein expression, and Marius Clore and Dennis Torchia for useful discussions. This work was supported by the AIDS Targeted Anti-Viral Program of the Office of the Director of the National Institutes of Health.


πŸ“œ SIMILAR VOLUMES


Correlation of Backbone Amide and Side-C
✍ Frank LΓΆhr; Heinz RΓΌterjans πŸ“‚ Article πŸ“… 2002 πŸ› Elsevier Science 🌐 English βš– 177 KB

Side-chain carbon resonance assignments are difficult to obtain for larger proteins. While standard methods require protons for excitation and detection of magnetization, their presence is often unacceptable and often leads to unacceptable relaxation losses at the directly bound carbon sites. In thi

Correlation of the15N(i+ 1),13CΞ±(i), and
✍ Klaas Dijkstra; Gerard J.A. Kroon; Eiso AB; Ruud M. Scheek; Johan Kemmink πŸ“‚ Article πŸ“… 1997 πŸ› Elsevier Science 🌐 English βš– 107 KB

Recently, a novel three-dimensional, triple-resonance ex-respect to the nitrogen, which then evolves into in-phase periment was described which correlates intraresidue 13 CO, carbonyl magnetization again. Evolution under the 1 J CaCO 13

HCN, A Triple-Resonance NMR Technique fo
✍ James L. Sudmeier; Elissa L. Ash; Ulrich L. GΓΌnther; Xuelian Luo; Peter A. Bullo πŸ“‚ Article πŸ“… 1996 πŸ› Elsevier Science 🌐 English βš– 299 KB

HCN, a new 3D NMR technique for stepwise coherence transfer rarely sufficient to establish the mechanism through which from 1 H to 13 C to 15 N and reverse through direct spin couplings it participates in the protein's function. The structure may 1 J CH and 1 J CN , is presented as a method for dete