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Ultrarapid freezing (URF) of mouse and human preembryos: A modified approach

✍ Scribed by Patricia E. Barg; David H. Barad; Wilfried Feichtinger


Book ID
105084235
Publisher
Springer US
Year
1990
Tongue
English
Weight
227 KB
Volume
7
Category
Article
ISSN
1573-7330

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## Background: We sought to determine whether more than one freeze/thaw cycle is required and what minimum temperature reliably kills prostate cancer in vivo. ## Methods: Two human prostate cancer cell lines (lncap and pc3) were implanted subcutaneously in male nude balb/c mice. tumors were froze