Determination of optimal freezing parameters of human prostate cancer in a nude mouse model

✍ Scribed by Turk, T.M.T.; Rees, M.A.; Pietrow, P.; Myers, C.E.; Mills, S.E.; Gillenwater, J.Y.

• Publisher: John Wiley and Sons
• Year: 1999
• Tongue: English
• Weight: 177 KB
• Volume: 38
• Category: Article
• ISSN: 0270-4137
• DOI: 10.1002/(sici)1097-0045(19990201)38:2<137::aid-pros7>3.0.co;2-5

✦ Synopsis

Background:

We sought to determine whether more than one freeze/thaw cycle is required and what minimum temperature reliably kills prostate cancer in vivo.

Methods:

Two human prostate cancer cell lines (lncap and pc3) were implanted subcutaneously in male nude balb/c mice. tumors were frozen with contemporary cryosurgery equipment and monitored for temperature, size, and serum prostate-specific antigen (psa) measurements. the tumors were subjected to one or two freeze/thaw cycles through a wide range of temperatures from 0 - -80 degrees c.

Results:

These experiments show that a single freeze/thaw to a temperature < -40 degrees c is adequate to kill most tumor cells in this mouse model of prostate cancer.

Conclusions:

Freezing prostate cancer to < -40 degrees c and ensuring that the entire tumor is frozen is more important than additional freeze/thaw cycles in this experimental model.