Ultrafiltration in serum protein binding determinations
β Scribed by John B. Whitlam; Kenneth F. Brown
- Publisher
- John Wiley and Sons
- Year
- 1981
- Tongue
- English
- Weight
- 612 KB
- Volume
- 70
- Category
- Article
- ISSN
- 0022-3549
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π SIMILAR VOLUMES
The determination of serum total proteins by biuret reaction is subject to severe lightscattering interference in the presence of elevated triglycerides. In addition, the dilution effect due to the voluminous character of certain lipoproteins creates an apparent diminished concentration of the serum
The binding of 99mTc(Sn)-MDP to human serum albumin and other blood proteins was investigated by gel chromatography and ultrafiltration. During gel chromatography dissociation of the 99mTc(Sn)-MDP-protein complex occurs: thus, it is not a suitable technique for the determination of protein binding.