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Two modes of loss of the tol function from Pseudomonas putida mt-2

โœ Scribed by Bayley, Susan A. ;Duggleby, Clive J. ;Worsey, Michael J. ;Williams, Peter A. ;Hardy, Kimber G. ;Broda, Paul

Book ID
104725346
Publisher
Springer
Year
1977
Tongue
English
Weight
176 KB
Volume
154
Category
Article
ISSN
0026-8925

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โœฆ Synopsis

Some of a set of independently arising Tol- (non toluate-utilising) derivatives of Pseudomonas putida mt-2 have lost the unique plasmid present in the parent strain. In others this plasmid has suffered a deletion of a specific region of about 27 Md.

๐Ÿ“œ SIMILAR VOLUMES

A cleavage map of the TOL plasmid of Pse
A cleavage map of the TOL plasmid of Pseudomonas putida mt-2
โœ Downing, Robert ;Broda, Paul ๐Ÿ“‚ Article ๐Ÿ“… 1979 ๐Ÿ› Springer ๐ŸŒ English โš– 240 KB

A cleavage map of the TOL plasmid pWWO has been determined for the restriction endonucleases HindIII and XhoI. A number of techniques were employed including (i) digestion of purified cleavage products with a second enzyme; (ii) hybridisation of purified XhoI fragments to Southern blots of HindIII d

An endonuclease cleavage map of the plas
An endonuclease cleavage map of the plasmid pWWO-8, a derivative of the TOL plasmid of Pseudomonas putida mt-2
โœ Downing, Robert G. ;Duggleby, Clive J. ;Villems, Richard ;Broda, Paul ๐Ÿ“‚ Article ๐Ÿ“… 1979 ๐Ÿ› Springer ๐ŸŒ English โš– 258 KB

Cleavage sites on the pWWO-8 plasmid were determined for the restriction endonucleases HindIII and XhoI. Terminal labelling using DNA polymerase I was particularly useful both for the characterisation of the smaller cleavage products and for confirmation of the order of fragments in the intact plasm