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A cleavage map of the TOL plasmid of Pseudomonas putida mt-2

โœ Scribed by Downing, Robert ;Broda, Paul

Book ID
104692894
Publisher
Springer
Year
1979
Tongue
English
Weight
240 KB
Volume
177
Category
Article
ISSN
0026-8925

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โœฆ Synopsis

A cleavage map of the TOL plasmid pWWO has been determined for the restriction endonucleases HindIII and XhoI. A number of techniques were employed including (i) digestion of purified cleavage products with a second enzyme; (ii) hybridisation of purified XhoI fragments to Southern blots of HindIII digest products and (iii) analysis of a number of deletion mutants.

๐Ÿ“œ SIMILAR VOLUMES

An endonuclease cleavage map of the plas
An endonuclease cleavage map of the plasmid pWWO-8, a derivative of the TOL plasmid of Pseudomonas putida mt-2
โœ Downing, Robert G. ;Duggleby, Clive J. ;Villems, Richard ;Broda, Paul ๐Ÿ“‚ Article ๐Ÿ“… 1979 ๐Ÿ› Springer ๐ŸŒ English โš– 258 KB

Cleavage sites on the pWWO-8 plasmid were determined for the restriction endonucleases HindIII and XhoI. Terminal labelling using DNA polymerase I was particularly useful both for the characterisation of the smaller cleavage products and for confirmation of the order of fragments in the intact plasm

Two modes of loss of the tol function fr
Two modes of loss of the tol function from Pseudomonas putida mt-2
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Some of a set of independently arising Tol- (non toluate-utilising) derivatives of Pseudomonas putida mt-2 have lost the unique plasmid present in the parent strain. In others this plasmid has suffered a deletion of a specific region of about 27 Md.