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An endonuclease cleavage map of the plasmid pWWO-8, a derivative of the TOL plasmid of Pseudomonas putida mt-2

✍ Scribed by Downing, Robert G. ;Duggleby, Clive J. ;Villems, Richard ;Broda, Paul

Book ID: 104693282
Publisher: Springer
Year: 1979
Tongue: English
Weight: 258 KB
Volume: 168
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00267938

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✦ Synopsis

Cleavage sites on the pWWO-8 plasmid were determined for the restriction endonucleases HindIII and XhoI. Terminal labelling using DNA polymerase I was particularly useful both for the characterisation of the smaller cleavage products and for confirmation of the order of fragments in the intact plasmid.

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