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Two-dimensional electrophoresis analysis of proteins extracted fromAlexandriumsp. LC3

✍ Scribed by Hao Li; Jinlai Miao; Fengxia Cui; Guangyou Li


Book ID
107510125
Publisher
SP Science Press
Year
2007
Tongue
English
Weight
353 KB
Volume
6
Category
Article
ISSN
1672-5182

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Extraction of plant proteins for two-dim
✍ Dr. Fabienne Granier πŸ“‚ Article πŸ“… 1988 πŸ› John Wiley and Sons 🌐 English βš– 684 KB

Three different extraction procedures for two-dimensional electrophoresis of plant proteins are compared: (i) extraction of soluble proteins with a nondenaturing Tris-buffer, (ii) denaturing extraction in presence of sodium dodecyl sulfate at elevated temperature allowing the solubilization of membr