๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

Tumour angiogenesis factor (TAF) in human and animal tumours

โœ Scribed by Paul Phillips; James K. Steward; Shant Kumar

Publisher
John Wiley and Sons
Year
1976
Tongue
French
Weight
863 KB
Volume
17
Category
Article
ISSN
0020-7136

No coin nor oath required. For personal study only.

โœฆ Synopsis

Abstract

Extracts were made from Walker 256 carcinoma, spontaneous rat mammary adenocarcinoma, Wilms' tumour, human neuroblastoma and human haemangioma. Chromatography of the extracts on Sephadex Gโ€100 yielded four fractions, A, B, C and D. Injection of fractions B and C resulted in the growth of new capillaries in the subcutaneous fascia of rats. Controls, e.g. similar extracts of rat liver or human kidney, did not induce neovascularisation. The endothelium of newlyโ€formed blood vessels contained many mitotic figures. A limitation of this method is that it is qualitative only. In order to develop a quantitative in vitro assay for a tumour angiogenesis factor (TAF), shortโ€term primary cultures were initiated from adult rat brain white matter, as cells from such cultures were shown to be vascular in origin. Addition of fractions containing TAF (B and C) which were active in vivo failed to stimulate thymidine uptake by the cells. The possible reasons for this failure and the therapeutic potential of TAF in cancer control are discussed.

๐Ÿ“œ SIMILAR VOLUMES

Tumour angiogenesis factor (TAF) and its
Tumour angiogenesis factor (TAF) and its neutralisation by a xenogeneic antiserum
โœ Paul Phillips; Shant Kumar ๐Ÿ“‚ Article ๐Ÿ“… 1979 ๐Ÿ› John Wiley and Sons ๐ŸŒ French โš– 626 KB

## Abstract Tumour angiogenesis factor (TAF) has been extracted from a variety of animal and human tumours. Its ability to induce neovascularization in two bioassays, viz. rat airโ€sac and chick chorioโ€allantoic membrane, is demonstrated. Similar extracts from normal tissues fail to induce angiogene

Quantitation of extracts containing tumo
Quantitation of extracts containing tumour angiogenesis factor (TAF) by radioimmunometric and radioimmunoassays
โœ Ana M. Schor; S. Kumar; P. J. Phillips ๐Ÿ“‚ Article ๐Ÿ“… 1980 ๐Ÿ› John Wiley and Sons ๐ŸŒ French โš– 680 KB

## Abstract An antiserum which is able to inhibit TAFโ€induced neovascularization in vivo(TAF antiserum) was used to develop two quantitative assays for TAFโ€containing tumour extracts (tumor TAF).I) Radioimmunometric assay (RIMA): the IgG of the TAF antiserum was labelled with ^125^I. An excess of ^

Human lung tumour cell line adapted to g
Human lung tumour cell line adapted to grow in serum-free medium secretes angiogenesis factor
โœ S. Kumar; D. West; Mary Daniel; Anne Hancock; T. Carr ๐Ÿ“‚ Article ๐Ÿ“… 1983 ๐Ÿ› John Wiley and Sons ๐ŸŒ French โš– 490 KB

## Abstract A human lung tumour cell line has been grown in serumโ€free medium for 12 months and during this period cells were subcultured every 4 days. The culture medium contained an angiogenesis factor which has been purified and shown to be similar to that isolated from other sources including s

Mast cells and tumour angiogenesis: The
Mast cells and tumour angiogenesis: The tumour-mediated release of an endothelial growth factor from mast cells
โœ William R. Roche ๐Ÿ“‚ Article ๐Ÿ“… 1985 ๐Ÿ› John Wiley and Sons ๐ŸŒ French โš– 780 KB

Exposure to tumour cells has previously been shown to induce mast cells to degranulate and release heparin. Isolated mastsell granules were found to be mitogenic for endothelial cells in vitro. This effect was a property of mast-cell heparin, whose potency as a mitogen exceeded that of commercial he