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Tumor necrosis factor α drives cadherin 11 expression in rheumatoid inflammation

✍ Scribed by Bernard Vandooren; Tineke Cantaert; Mariëtte Ter Borg; Troy Noordenbos; Rodger Kuhlman; Daniëlle Gerlag; Tim Bongartz; Kris Reedquist; Paul P. Tak; Dominique Baeten

Publisher
John Wiley and Sons
Year
2008
Tongue
English
Weight
323 KB
Volume
58
Category
Article
ISSN
0004-3591

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✦ Synopsis

Abstract

Objective

Cadherin 11 expressed on fibroblast‐like synoviocytes (FLS) plays a key role in normal synovial architecture. The purpose of this study was to examine the expression of cadherin 11 in human synovitis.

Methods

Cadherin 11 expression in synovial biopsy samples from patients with various types of arthritis and in lung biopsy samples from patients with interstitial pneumonitis (IP) was examined by immunostaining. The regulation of cadherin 11 expression in human FLS was assessed by quantitative reverse transcription–polymerase chain reaction analysis and Western blotting. Therapeutic modulation of synovial cadherin 11 was assessed before and after effective antiinflammatory therapy.

Results

Abundant staining for cadherin 11 was seen in the intimal lining layer and the synovial sublining in inflamed tissues, with discrete staining in noninflammatory osteoarthritic (OA) tissues. The pattern and degree of immunostaining were similar in tissues from patients with rheumatoid arthritis (RA), nonpsoriatic spondylarthritis (SpA), psoriatic arthritis (PsA), and inflammatory OA. Clear staining for cadherin 11 was also observed in lung tissues from RA‐associated IP and idiopathic IP patients, but was very limited in normal lung tissue. Cadherin 11 staining correlated strongly with the degree of inflammatory infiltration of the tissue, as well as with the C‐reactive protein level and the erythrocyte sedimentation rate in RA patients. In vitro, cadherin 11 expression by FLS was consistently up‐regulated by tumor necrosis factor α (TNFα) at the protein, but not the messenger RNA, level. Cadherin 11 staining in vivo was strongly down‐regulated by prednisone treatment in RA patients and by TNFα blockade in SpA patients.

Conclusion

Cadherin 11 expression is regulated by mediators of inflammation, such as TNFα. Since cadherin 11 plays an important role in cartilage destruction in experimental arthritis, down‐modulation of cadherin 11 by potent antiinflammatory therapies in humans with arthritis may contribute to halting cartilage damage.

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