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Trichloroacetic Acid-Induced Molten Globule State of Aminoacylase from Pig Kidney

✍ Scribed by Zhen Xu; Qiang Xie; Hai-Meng Zhou

Book ID
114421926
Publisher
Springer
Year
2003
Tongue
English
Weight
96 KB
Volume
22
Category
Article
ISSN
1573-4943

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## Abstract Titration of a salt‐free solution of native staphylococcal nuclease by HC1 leads to an unfolding transition in the vicinity of pH 4, as determined by near‐ and far‐UV circular dichroism. At pH 2‐3, the protein is substantially unfolded. The addition of further HC1 results in a second tr