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Acid-Induced Molten Globule State of a Fully Active Mutant of Human Interleukin-6 †

✍ Scribed by De Filippis, Vincenzo; Polverino de Laureto, Patrizia; Toniutti, Nicoletta; Fontana, Angelo


Book ID
126265281
Publisher
American Chemical Society
Year
1996
Tongue
English
Weight
430 KB
Volume
35
Category
Article
ISSN
0006-2960

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## Abstract Titration of a salt‐free solution of native staphylococcal nuclease by HC1 leads to an unfolding transition in the vicinity of pH 4, as determined by near‐ and far‐UV circular dichroism. At pH 2‐3, the protein is substantially unfolded. The addition of further HC1 results in a second tr