Characterization of Acid-Induced Partially Folded Conformation Resembling a Molten Globule State of Polygalacturonase from a Filamentous FungusTetracoccosporiumsp.

Characterization of the stable, acid-ind

Characterization of the stable, acid-induced, molten globule-like state of staphylococcal nuclease

✍ Anthony L. Fink; Linda J. Calciano; Yuji Goto; Midori Nishimura; Sally A. Swedbe 📂 Article 📅 1993 🏛 Cold Spring Harbor Laboratory Press 🌐 English ⚖ 569 KB

## Abstract Titration of a salt‐free solution of native staphylococcal nuclease by HC1 leads to an unfolding transition in the vicinity of pH 4, as determined by near‐ and far‐UV circular dichroism. At pH 2‐3, the protein is substantially unfolded. The addition of further HC1 results in a second tr