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Tributyltin is a potent inducer of the heat shock response in human diploid fibroblasts

✍ Scribed by Hua Zhang; Alice Y.-C. Liu

Publisher: John Wiley and Sons
Year: 1992
Tongue: English
Weight: 723 KB
Volume: 153
Category: Article
ISSN: 0021-9541
DOI: 10.1002/jcp.1041530304

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✦ Synopsis

Submicromolar concentrations of tributyltin (TBT), a commercially used organotin compound, were found to induce the expression of several stress proteins, most notably HSP89 and HSP70, in IMK-90 human diploid fibroblasts in a time-and dose-dependent manner. This induction can be demowtrated by quantitation of 1) synthesis of the heat 5hock proteins (HSPs), 2) relative abundance of niRNA of hsp70, arid 3 ) Lransient expression of a human hsp70 promoter driven reporter gene. TBT also increased the abundance of mRNA of heme oxygenase, whereas heat shock was without effect. Analysis of protein binding to a consensus heat shock element (HSE) by electrophoretic mobility shift assay suggests that the induction of the heat shock response by TBT was attributable to activation of the heat shock transcription factor (HSTF).

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