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Transport of methotrexate in chinese hamster ovary cells: A mutant defective in methotrexate uptake and cell binding

โœ Scribed by Wayne F. Flintoff; Charles R. Nagainis


Book ID
115705913
Publisher
Elsevier Science
Year
1983
Tongue
English
Weight
643 KB
Volume
223
Category
Article
ISSN
0003-9861

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Methotrexate-poly(lysine) as a selective
โœ Hugues J.-P. Ryser; Richard Mandel; Asteghik Hacobian; Dr. Wei-Chiang Shen ๐Ÿ“‚ Article ๐Ÿ“… 1988 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 982 KB

Methotrexate (MTX) covalently linked to poly(L-lysine) [poly(Lys)] enters cells by endocytosis, is degraded in lysosomes and, upon liberation of small molecular methotrexate, is cytocidal to Chinese hamster cells in culture. This drug conjugate was used to select mutants resistant to MTX-poly(Lys),