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Transport and accumulation of purine bases by Crithidia fasciculata

✍ Scribed by G. W. Kidder; Virginia C. Dewey; Linda L. Nolan

Publisher
John Wiley and Sons
Year
1978
Tongue
English
Weight
457 KB
Volume
96
Category
Article
ISSN
0021-9541

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✦ Synopsis

Abstract

The capacity for the rapid transport of purine bases by Crithidia fasciculata is found only in cells starved for purines. Cells grown in complete medium transport poorly. Rapid transport capability appears and then disappears during growth of purine‐depleted cultures. This rapid transport appears to occur by a process of mediated diffusion. Two mechanisms are involved, one of low velocity and high affinity, the other of high velocity and low affinity. Accumulation of the bases within the cell occurs by their rapid conversion to ribonucleotides by phosphoribosyltransferases.

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