Analysis of purine and pyrimidine bases, ribonucleosides, and ribonucleotides by high-pressure liquid chromatography
โ Scribed by Erkki Nissinen
- Publisher
- Elsevier Science
- Year
- 1980
- Tongue
- English
- Weight
- 642 KB
- Volume
- 106
- Category
- Article
- ISSN
- 0003-2697
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## Abstract The separation of 12 model compounds chosen among purine and pyrimidine bases and nucleosides was studied by using hydrophilic interaction chromatography (HILIC). The compounds investigated were small molecules with relevant properties for biomedical and pharmaceutical studies. The mixt
Nucleotides, nucleosides, and purine bases were extracted from human endomyocardial biopsies, freeze-clamped rat hearts, and porcine coronary sinus plasma. Perchloric acid extracts were neutralized with Freon-trioctylamine and analyzed at 250 nm by reverse-phase ion-pairing high-performance liquid c
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