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Transmission of mitochondrial DNA inUstilago violacea

✍ Scribed by Gerhard Wilch; Steven Ward; Alan Castle

Book ID
104735300
Publisher
Springer-Verlag
Year
1992
Tongue
English
Weight
730 KB
Volume
22
Category
Article
ISSN
0172-8083

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✦ Synopsis

Mitochondrial DNA (mtDNA) restriction fragment length polymorphisms (RFLPs) were used as genetic markers for following mitochondrial transmission in the basidiomycete Ustilago violacea. Yeast-like cells of opposite mating types (a1 and a2) were mated on 2% water agar and were treated with alpha-tocopherol to induce formation of dikaryotic hyphae. Upon depletion of the alpha-tocopherol, the hyphae budded off haploid cells with parental nuclear genotypes. These cells were examined for mitochondrial RFLP phenotype. In progeny expressing the a1 mating type, mitochondria from either parent were observed equally frequently. In progeny with the a2 mating type, mitochondria were almost exclusively (94%) from the a2 parent.

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