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Transmission electron microscopy of the cytoskeleton of migratory and invasive cells

✍ Scribed by McGarvey, Terence ;Persky, Bruce

Publisher: Wiley (John Wiley & Sons)
Year: 1989
Tongue: English
Weight: 310 KB
Volume: 13
Category: Article
ISSN: 0741-0581
DOI: 10.1002/jemt.1060130315

No coin nor oath required. For personal study only.

✦ Synopsis

INTRODUCTION.

The recent development of Transwell cell culture chamber inserts (Costar Co., Cambridge, MA; cat. nos. 3421 & 3422) permits analysis of cellular migration and invasion. Transwell inserts contain a porous polycarbonate membrane and fit into 6 and 24 well cluster plates. Although cellular migration and invasion through polycarbonate membranes have been quantitated by routine LM, SEM and radiolabel techniques, few reports have focused on the cytoskeleton of cells in the process of moving through pores. This communication reports a methodology to evaluate the cytoskeleton of both migratory and invasive tumor cells within 5 and 8 um diameter pore Transwell membranes.

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