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A simple technique for the visualization of whole mount cytoskeletons with transmission electron microscopy

โœ Scribed by Marek, Louis F. ;Kelley, Robert O.

Publisher
John Wiley and Sons
Year
1983
Tongue
English
Weight
870 KB
Volume
207
Category
Article
ISSN
0003-276X

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โœฆ Synopsis

Abstract

Examination of whole mount cells in the transmission electron microscope has been useful in studies of cellular architecture. The common technique is to grow cells directly on formvarโ€coated, gold grids for direct observation through a cell. We report a technique for obtaining whole mount preparations which requires neither fragile formvar films nor expensive, gold grids. Cells are grown on palladiumโ€coated coverslips and processed for electron microscopy. The cells and the palladium substrate are separated from the coverslip. The cellโ€palladium complex is then picked up on copper grids as in thin section processing. We compare images of the cytoskeleton using our technique with images using previously described techniques and present preliminary observations of contracting cell models. Such contractions would tear formvar films if attempted on cells grown in the conventional manner for whole mount examination. Our technique allows cells to contract without tearing the underlying substrate.

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