Subtilisin-A was colyophilized with various types of support materials, such as Amberlite IRC-50, Celite545, chitosan, DEAE-cellulose, DOWEX-1, zeolite, glass bead, and polystyrene. The colyophilized enzyme was used for the optical resolution of racemic l-phenylethylamine with 2,2,2-trifluoroethylbu
Transition state stabilization of subtilisins in organic media
β Scribed by Zu-Feng Xu; Rhett Affleck; Pramod Wangikar; Valerie Suzawa; Jonathan S. Dordick; Douglas S. Clark
- Publisher
- John Wiley and Sons
- Year
- 1994
- Tongue
- English
- Weight
- 556 KB
- Volume
- 43
- Category
- Article
- ISSN
- 0006-3592
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## Abstract Colyophilization or codrying of subtilisin Carlsberg with the crown ethers 18βcrownβ6, 15βcrownβ5, and 12βcrownβ4 substantially improved enzyme activity in THF, acetonitrile, and 1,4βdioxane in the transesterification reactions of __N__βacetylβLβphenylalanine ethylester and 1βpropanol a